Carbon-14 dating by AMS

Carbon-14 dating by AMS

Carbon-14 dating, the benchmark in archaeometry, is performed using particle accelerators coupled with mass spectrometry (AMS) to date archaeological organic materials weighing more than 3 mg.

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Close-up of a tree illustrating carbon-14 dating using accelerator mass spectrometry

C14 dating by AMS,
a rapid and reliable method

The AMS method (accelerator mass spectrometry) allows for dating organic archaeomaterials but also for analyzing ancient mortars. Thanks to cutting-edge technologies, our scientists obtain precise and rigorous results, enabling the establishment of a chronology of stratigraphies.

Aware of the constraints of preventive archaeology projects, we guarantee a turnaround time of 10 working days to provide dating results and their calibration.

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Close-up of a bone sample

The role of carbon-14 in dating archaeological objects

The carbon-14, also called radiocarbon, is a radioactive isotope of carbon, in the sense that it is unstable (it will transform over time). All living organisms possess this isotope. During the organism's life, the concentration of carbon-14 will be constant. As soon as an organism dies, the amount of carbon-14 decreases proportionally over time. Its concentration halves every 5730 years.

Accelerator Mass Spectrometry (AMS) is an advanced technology requiring only 1 mg of pure carbon. It offers an accuracy of ±25 to 35 years on the raw age, and the complete analysis takes only a few hours.

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Close-up of a fossil

AMS carbon-14-dated materials

AMS carbon-14 datingis one of our specialties. Thanks to this method, our laboratories are able to date a wide range of organic materials:

  • Wood and charcoal,
  • Bones and teeth,
  • Seeds and organic residues,
  • Shells, peat, etc.

Before each measurement, samples undergo chemical pre-treatments to remove contaminants and extract the datable fractions (cellulose for wood, collagen for bone, etc.). Our teams adapt these processes according to the material.

The carbon will then need to be extracted to create a graphite target, and the stable isotopes of carbon and nitrogen will be analyzed. A complete analysis will be carried out, including raw age and calibrated dates. The results will then be compiled and commented on in a study report.

Despite these numerous steps, the CIRAM laboratories guarantee a turnaround time of 10 working days to provide dating results.

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Stages of carbon-14 dating in archaeology

01 - Processing and extraction

Prior to dating, samples must be purified to remove contaminants. We apply ABA (Acid-Base-Acid) treatment, which consists of :

  • Hydrochloric acid decontamination to remove surface impurities.
  • Wash with demineralized water and dry.
  • Treatment with sodium hydroxide to eliminate humic and fulvic acids.
  • A second hydrochloric acid bath to prevent absorption of atmospheric CO₂.

As for the bones, they undergo a series of preliminary treatments to extract the collagen. Here's how it works:

  • Hydrochloric acid treatment to remove surface contamination and partially deteriorate the mineral part of the bone.
  • Wash with demineralized water.
  • Treatment with sodium hydroxide to eliminate humic and fulvic acids.
  • Extraction of collagen by heat treatment with hydrochloric acid.
  • Filtration and rinsing to recover purified collagen.

02 - Graphitization and stable isotopes

After the treatments and extractions, it is necessary to extract the carbon from the other elements: this is the graphitization stage. In this stage, the sample is burnt to produce CO₂, which is then reduced to graphite, a solid pure carbon. At the same time, the quality of the collagen is checked by measuring the stable isotopes of carbon and nitrogen using an isotope ratio mass spectrometer (IRMS) and determining the C/N ratio with an elemental analyzer.

Since 2018, over 20,000 samples have been analyzed. CIRAM laboratories have two automated analysis and graphitization lines, each including

  • An Elementar Vario Isotope select elemental analyzer for sample combustion and C/N ratio determination,
  • An Elementar IRMS mass spectrometer for the analysis of stable carbon and nitrogen isotopes (𝛿13C and 𝛿15N),
  • Two AGE3 Ions Plus automated graphitizing systems to transform samples into graphite for AMS measurement.

03 - Dating bioapatite

Some bones, especially those burned or preserved in arid environments, no longer contain collagen or are too degraded for reliable dating.

To assess collagen conservation, we measure the C/N (carbon/nitrogen) ratio. If this ratio is between 2.9 and 3.6, classical carbon-14 dating on collagen is possible. On the other hand, if this threshold is not met, dating must be carried out on bioapatite, the mineral part of the bones.

In this case, the carbon dioxide contained in the bioapatite is extracted by reaction with phosphoric acid, then cryogenically purified to be isolated from the other elements. Finally, this CO₂ is transformed into graphite for analysis by Accelerator Mass Spectrometry (AMS).

CIRAM, specialist in carbon-14 dating by AMS

CIRAM is a leader in AMS radiocarbon dating and the analysis of archaeomaterials. Our laboratories, equipped with the latest technologies, also carry out analyses in anthracology/xylology, organic residues, luminescence dating (TL/OSL), and isotopic analysis.

By following rigorous protocols similar to those of public laboratories, we guarantee reliable, precise, and rapid results, adapted to the requirements of preventive archaeology.

Our scientists do not just provide physical measurements: we also offer a detailed interpretation of the results, taking into account the specific archaeological context and your issues.

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Are you asking yourself these questions?

What types of materials can be carbon-14 dated?

Any organic material can be dated, such as wood, charcoal, bones, teeth, seeds, organic residues, peat, shells and so on.

How much material is needed for an analysis?

This depends on the type of sample. For example:

Materials Minimum quantity required Optimum quantity recommended
Wood 20 mg 100 mg
Charcoal 20 mg 100 mg
Organic residue 20 mg 100 mg
Seed 20 mg 100 mg
Shell 50 mg 100 mg
Beachrock 50 mg 100 mg
Peat 50 mg 100 mg
Bone, tooth, ivory 0.5 g 1 g
Burnt bone 2 g 3 g
How do I send a sample for analysis?

We provide a detailed shipping protocol to ensure samples are preserved until analysis.

What are the deadlines for carbon-14 dating in archaeology?

We guarantee results within 10 working days of receipt of samples.

Can carbon-14 dating be used in art and industry?

Yes, it can also be used to authenticate works of art and quantify the biomass content of industrial materials.

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